Studies Supporting Effectiveness of FLORISH™️ Spore Probiotic with Fulvic Acid

The various research papers, studies and clinical trails that have been done and include the findings and recommendations which have reference to the products we endorse and promote have been outlined below for your easy reference and additional information.

We have participated in many research studies locally and internationally where our supplements have been used to treat patients in different programmes and protocols.

One of the most widely used studies, and the one that we most often refer to from our social media and print publications can be found right here.

Download the LPS-ME Leaky Gut Research Paper

There are many other links below that outline the other research resources to use.


Positive mood enhancing ability of probiotics

Reduces inflammation

Reduction in “leaky gut” resulting in reduction of endotoxicity and inflammation

Bacillus subtilis/lichenformis mix reprograms the gut microbiota

The following study demonstrates increase in microbial diversity in the gut as a result of using Bacillus probiotics found in FLORISH

Oral Administration of a Select Mixture of Bacillus Probiotics Affects the Gut Microbiota and Goblet Cell Function 

Wei Zhang,a Yao-Hong Zhu,a Dong Zhou,a Qiong Wu,et al

Alkalising effects of Bacillus lichenformis - reducing acidosis

Protease production,%20et%20al.pdf

Vitamin B production in the gut by Bacillus lichenformis,%20et%20al.pdf

Bacillus indicus producing carotenoids in the gut

Antibiotic resistance of Bacillus clausii

Positively affects and reduces “leaky gut” reducing LPS leakage into circulatory system, reducing inflammation in the body through endotoxicity

Oral spore-based probiotic supplementation was associated with reduced incidence of post-prandial dietary endotoxin, triglycerides, and disease risk biomarkers

AUTHOR(s) Brian K McFarlin, Andrea L Henning, Erin M Bowman, et al


Production of natto, vitamin K2 7 in the gut and facilitation of GALT (gut associated lymphoid tissue)

    Bacillus subtilis

    - Produces over 12 affective antibiotics - which target fungus’s and pathogens

    - Highly important for immune development - GALT (gut associated lymphatic tissue)

    - Produces nattokinase and vitamin K2


    Effect of Biofilm Formation by Bacillus subtilis natto on Menaquinone-7 Biosynthesis

    Aydin Berenjian

    Email author Natalie Li-Cheng ChanRaja MahanamaAndrea Et Al 

    Rhee KJ, Sethupathi P, Driks A, et al

    Role of commensal bacteria in development of gut-associated lymphoid tissue and preimmune antibody repertoire J Immunol. 2004;172(2):1118-24

    Stress factor in causing leaky gut syndrome inducing endotoxaemia resulting in low-grade inflammation

    De Punder K, Pruimboom L.

    Stress induces endotoxemia and low-grade inflammation by increasing barrier permeability. Front Immunol. 2015;15(6):223.

    Gong Y, Li H, Li Y.

    Effects of Bacillus subtilis on epithelial tight junctions of mice with inflammatory bowel disease. 

    J Interferon Cytokine Res. 2016;36(2). 

    Samanya M, Yamauchi K.

    Histological alterations of intestinal villi in chickens fed dried Bacillus subtilis var. natto. 

    Comp Biochem Physiol A Mol Integr Physiol. 2002;133(1):95-104.

    Tam NKM, Uyen NQ, Hong HA, et al

    The Intestinal Life Cycle of Bacillus subtilis and Close Relatives. 

    Journal of Bacteriology. 2006;188(7):2692-2700.

    • Immune stimulation of Bacillus subtilis

    Lefevre M, Racedo SM, Ripert G, et al

    Probiotic strain Bacillus subtilis CU1 stimulates immune system of elderly during common infectious disease period: a randomized, double-blind placebo-controlled study. Immun Aging. 2015;12:24.

    Serra CR, Earl AM, Barbosa TM, et al

    Sporulation during Growth in a Gut Isolate of Bacillus subtilis. J Bacteriol. 2014;196(23):4184-4196. 

    Hong HA, Khaneja R, Tam NMK, et al.

    Bacillus subtilis isolated from the human gastrointestinal tract. Res Microbiol. 2009;160(2):134-143. 

    Mandel DR, Eichas K, Holmes J.

    • Using FLORISH is a viable therapy for relieving symptoms of rheumatoid arthritis

    Bacillus coagulans: a viable adjunct therapy for relieving symptoms of rheumatoid arthritis according to a randomized, controlled trial. BMC Complement Altern Med. 2010;10:1.

    • Using FLORISH will help prevent recurrent respiratory infections in children

    Marseglia GL, Tosca M, Cirillo I, et al.

    Efficacy of Bacillus clausii spores in the prevention of recurrent respiratory infections in children: a pilot study Ther Clin Risk Manag. 2007;3(1):13-7.

    Urdaci MC, Bressollier P, Pinchuk I, et al

    Bacillus clausii probiotic strains: antimicrobial and immunomodulatory activities. J Clin Gastroenterol. 2004;38(6 Suppl):S86-90.

    Nista EC, Candelli M, Cremonini F, et al

    Bacillus clausii therapy to reduce side-effects of anti-Helicobacter pylori treatment: randomized, double-blind, placebo controlled trial. Aliment Pharmacol Ther. 2004 Nov;20(10):1181-8.

    • FLORISH is an effective treatment for SIBO (small intestinal bacterial overgrowth)

    Gabrielli M, Lauritano EC, Scarpellini E, et al

    Bacillus clausii as a Treatment of Small Intestinal Bacterial Overgrowth. Am J Gastroenterol. 2009;104(5):1327-8.

    Di Caro S, Tao H, Grillo A, et al

    Bacillus clausii effect on gene expression pattern in small bowel mucosa using DNA microarray analysis. Eur J Gastroenterol Hepatol. 2005;17(9):951-60.

    Ciprandi G, Tosca MA, Milanese M, et al 

    Cytokines evaluation in nasal lavage of allergic children after Bacillus clausii administration: A pilot study. Pediatr Allergy Immunol. 2004;15(2):148-51.

    • Spore Probiotics Able to Survive Gastric Passage

    “Survival of Probiotics in Simulated Gastric Fluid.”

    Food Science Center Report. Silliker Labs. RPN 16663. August 24, 2013. 

    Gibson GR, Rouzaud G, Brostoff J, et al

    An evaluation of probiotic effects in the human gut: microbial aspects. Final Technical report. FSA project ref G01022.

    Casula G, Cutting SM.

    Bacillus Probiotics: Spore Germination in the Gastrointestinal Tract. App Environ Microb. 2002;68(5):2344-2352.



    Check out the Sebastian Says YouTube Channel for some more informations and interesting insights from the creator of FLORISH Spore Probiotic and FULFIXER Fulvic Acid.